Problem of resistant bacteria to disinfectants

The work is carried out in cooperation with the Laboratory of Anatomy of microorganisms of Institute of Epidemiology and Microbiology named after N.F.Gamaley, Scientific and Research Institute of Rospotrebnadzor (Federal Service for Consumer Rights Protection and Human Welfare) and State Scientific Center of Applied Microbiology and Biotechnology of Rospotrebnadzor.

The widespread use of the so-called sub-bactericidal concentrations of working solutions of disinfectants leads to the development of acquired bacterial resistance to disinfectants.

As a result of research conducted in the laboratory of environment of State Scientific Center of Applied Microbiology and Biotechnology of Rospotrebnadzor there was proved the existence of so-called associated or induced bacterial resistance to antibiotics as a result of the occurrence of acquired resistance to disinfectants.

The main objective of research is to study the mechanisms of the effects of certain active substances of disinfectants on bacterial cells and limiting of the development of bacteria resistance.

Currently elucidated mechanisms of action of tertiary alkyl amines (for example, drug OPTIMAKS) on gram-positive and gram-negative bacteria are found.

The target for the attack of tertiary alkylamines molecules are phosphate groups of biological molecules - proteins, phospholipids, DNA, RNA. It is shown that under the influence of tertiary alkylamines on vegetative (planktonic) bacteria flocculation occurs with the formation of multicellular conglomerates, the destruction of the cell wall and cytoplasmic membrane, total disorganization of the cytoplasm and ribonucleoprotein complex, the destruction of plasmids - extrachromosomal hereditary factors responsible for the emergence and spread of bacterial resistance.

Thus, the mechanism of action of a disinfectant "OPTIMAKS" prevents bacterial resistance.

The revealed mechanism of action of tertiary alkylamines on planktonic bacteria is an absolute novelty that was not published previously.